The city council and its partners made a bid for the city to be included in the programme after the Scottish Government announced its intention to introduce them last year.
The new zones will offer incentives — such as tax reliefs — to firms locating in certain areas of Scotland and creating new jobs.
The thrust of Dundee's bid was focused on the opportunities within the green energy sector and its associated supply chain.
Scotland's four new enterprise areas were confirmed on Tuesday by Finance Secretary John Swinney.
It explores options for a possible new manufacturing plant at Dundee Port that would create hundreds of highly-skilled posts.